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下載說明 ①Overview
標準曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 500.00 | 2.368 | 2.285 |
| 250.00 | 1.720 | 1.637 |
| 125.00 | 1.225 | 1.142 |
| 62.50 | 0.782 | 0.699 |
| 31.25 | 0.586 | 0.503 |
| 15.63 | 0.321 | 0.238 |
| 7.82 | 0.251 | 0.168 |
| 0.00 | 0.083 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-105% | 97% |
| EDTA plasma(n=5) | 85-97% | 91% |
| Heparin plasma(n=5) | 92-105% | 98% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-98% | 93-106% | 89-97% | 85-94% |
| EDTA plasma(n=5) | 82-96% | 85-98% | 95-104% | 82-96% |
| Heparin plasma(n=5) | 85-96% | 87-101% | 89-97% | 85-94% |
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