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人強(qiáng)啡肽(Dyn)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1675

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human Dyn(Big Dynorphin) ELISA Kit
Reactivity:	Human
Alternative Names:	B-DYN; Biocytin 13 Dynorphin A 1-13 Amide
Assay Type:	Competitive Inhibition
Sensitivity:	2.56 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Signal transduction;Neuro science;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Dyn protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Dyn. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Dyn in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	0.215
250.00	0.336
125.00	0.451
62.50	0.899
31.25	1.157
15.63	1.457
7.82	1.624
0.00	2.157

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Dyn and the recovery rates were calculated by comparing the measured value to the expected amount of Dyn in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	94-107%	100%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dyn and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.