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人補體成分4(C4)酶聯(lián)免疫吸附檢測試劑盒

ELK1653

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human C4(Complement Component 4) ELISA Kit
Reactivity:	Human
Alternative Names:	C4BP-a; Complement Component 4 Binding Protein,Alpha
Assay Type:	Sandwich
Sensitivity:	1.35 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Infection immunity;Hematology;
Uniprot ID:	P0C0L4
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
200.00	2.266	2.185
100.00	1.497	1.416
50.00	0.935	0.854
25.00	0.872	0.791
12.50	0.427	0.346
6.25	0.361	0.280
3.13	0.187	0.106
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant C4 and the recovery rates were calculated by comparing the measured value to the expected amount of C4 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-103%	96%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	84-98%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.