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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
6000.00 | 0.235 | |
3000.00 | 0.433 | |
1500.00 | 0.711 | |
750.00 | 0.967 | |
375.00 | 1.257 | |
187.50 | 1.565 | |
93.75 | 1.877 | |
0.00 | 2.197 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 85-98% | 91% |
Heparin plasma(n=5) | 96-107% | 102% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-104% | 97-106% | 86-97% | 92-99% |
EDTA plasma(n=5) | 85-93% | 87-96% | 79-91% | 95-103% |
Heparin plasma(n=5) | 88-102% | 81-96% | 86-93% | 82-95% |
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