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下載說明 ①Overview
標準曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.154 | 2.058 |
| 5.00 | 1.694 | 1.598 |
| 2.50 | 1.121 | 1.025 |
| 1.25 | 0.826 | 0.730 |
| 0.63 | 0.457 | 0.361 |
| 0.32 | 0.305 | 0.209 |
| 0.16 | 0.208 | 0.112 |
| 0.00 | 0.096 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant PPAR-γ and the recovery rates were calculated by comparing the measured value to the expected amount of PPAR-γ in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-105% | 97% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 83-95% | 89% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPAR-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-94% | 87-96% | 92-101% | 82-98% |
| EDTA plasma(n=5) | 87-92% | 85-94% | 89-98% | 85-94% |
| Heparin plasma(n=5) | 82-93% | 95-104% | 82-96% | 85-98% |
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