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下載說明 ①Overview
標準曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.126 | 2.041 |
| 5.00 | 1.628 | 1.543 |
| 2.50 | 1.128 | 1.043 |
| 1.25 | 0.847 | 0.762 |
| 0.63 | 0.527 | 0.442 |
| 0.32 | 0.335 | 0.250 |
| 0.16 | 0.179 | 0.094 |
| 0.00 | 0.085 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant PPAR-γ and the recovery rates were calculated by comparing the measured value to the expected amount of PPAR-γ in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 77-95% | 86% |
| EDTA plasma(n=5) | 80-97% | 88% |
| Heparin plasma(n=5) | 82-94% | 88% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPAR-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-98% | 91-103% | 85-97% | 87-96% |
| EDTA plasma(n=5) | 87-91% | 85-93% | 89-97% | 85-93% |
| Heparin plasma(n=5) | 89-97% | 85-94% | 87-96% | 85-97% |
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