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小鼠結(jié)合珠蛋白(HP)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1623

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Mouse HP(Haptoglobin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin; Hpt; Haptoglobin
Assay Type:	Sandwich
Sensitivity:	13.1 ng/mL
Standard:	2000 ng/mL
Detection Range:	31.25-2000 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Hematology;
Uniprot ID:	Q61646
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HP in the samples is then determined by comparing the OD of the samples to the standard curve.

Phytomedicine

Mechanistic study of fructus aurantii (Quzhou origin) in regulating ileal reg3g in the treatment for NASH

日期：2024-08-05 IF：6.7

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
2000.00	2.036	1.948
1000.00	1.682	1.594
500.00	1.128	1.040
250.00	0.839	0.751
125.00	0.594	0.506
62.50	0.319	0.231
31.25	0.202	0.114
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HP and the recovery rates were calculated by comparing the measured value to the expected amount of HP in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	92-107%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.