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人B-淋巴細(xì)胞激活抗原B7-2(LAB7-2)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1606

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human LAB7-2(B-Lymphocyte Activation Antigen B7-2) ELISA Kit
Reactivity:	Human
Alternative Names:	CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2
Assay Type:	Sandwich
Sensitivity:	0.274 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.131	2.036
5.00	1.563	1.468
2.50	1.251	1.156
1.25	0.896	0.801
0.63	0.453	0.358
0.32	0.335	0.240
0.16	0.230	0.135
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LAB7-2 and the recovery rates were calculated by comparing the measured value to the expected amount of LAB7-2 in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	85-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LAB7-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.