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        小鼠低氧誘導(dǎo)因子1α(HIF1a)酶聯(lián)免疫吸附檢測(cè)試劑盒
        
                    ELK1605
                    
        
                        
        
                            
        
                                規(guī)格：
                                價(jià)格：
                            
        
                                                        
        
                                48T
                                ￥1960.00
                            
        
                                                        
        
                                96T
                                ￥2800.00
                            
        
                                                    
        
                    
        
                    
                
        
            
        
        
        

                
        
            
        
                
        Overview
                    文獻(xiàn)
        
                
        
                    
        
                        
        
                            
        
                                
        
                                    
        
                                        Product name:
                                        Mouse HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
                                    
        
                                                                            
        
                                            Reactivity:
                                            Mouse
                                        
        
                                                                                
        
                                            Alternative Names:
                                            HIF1-Alpha; HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
                                        
        
                                                                                
        
                                           Assay Type:
                                           Sandwich
                                           
                                        
        
                                                                                
        
                                           Sensitivity:
                                           0.056 ng/mL
                                           
                                        
        
                                                                                
        
                                           Standard:
                                           10 ng/mL
                                           
                                        
        
                                                                                
        
                                           Detection Range:
                                           0.16-10 ng/mL
                                           
                                        
        
                                                                                
        
                                           Sample Type:
                                           serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
                                           
                                        
        
                                                                                
        
                                           Assay Length:
                                           3.5h
                                           
                                        
        
                                                                                
        
                                           Research Area:
                                           Tumor immunity;Cardiovascular biology;Reproductive science;
                                           
                                        
        
                                                                                
        
                                           Uniprot ID:
                                           Q61221
                                           
                                        
        
                                                                                
        
                                           Test principle:
                                           The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HIF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HIF1a in the samples is then determined by comparing the OD of the samples to the standard curve.
                                           
                                        
        
                                                                        
        
                            
        
                                                        
                                                    
        
                    
        
                
        
            
        
                        
        
                
        標(biāo)準(zhǔn)曲線
        
                
        
                    
        
                        
        
                            
        
                                Concentration (ng/mL)
                                OD
                                                                Corrected OD
                                                            
        
                                                        
        
                                10.00
                                2.401
                                                                2.318
                                                            
        
                                                        
        
                                5.00
                                1.779
                                                                1.696
                                                            
        
                                                        
        
                                2.50
                                1.194
                                                                1.111
                                                            
        
                                                        
        
                                1.25
                                0.831
                                                                0.748
                                                            
        
                                                        
        
                                0.63
                                0.563
                                                                0.480
                                                            
        
                                                        
        
                                0.32
                                0.313
                                                                0.230
                                                            
        
                                                        
        
                                0.16
                                0.252
                                                                0.169
                                                            
        
                                                        
        
                                0.00
                                0.083
                                                                0.000
                                                            
        
                                                    
        
                    
        

                    
        
                
        
            
        
                        
        
                
        精密度
        
                
        
                    
        Intra-assay Precision (Precision within an assay)：CV%<8%
        
                    
        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
        
                    
        Inter-assay Precision (Precision between assays)：CV%<10%
        
                    
        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
        
                
        
            
        
                        
        
                
        回收率
        
                
        
                    
        Matrices listed below were spiked with certain level of recombinant HIF1a and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1a in samples.
        
                    
        
                        
        
                            
        
                                Matrix
                                Recovery range
                                Average
                            
        
                            
        
                                serum(n=5)
                                79-93%
                                86%
                            
        
                            
        
                                EDTA plasma(n=5)
                                82-95%
                                88%
                            
        
                            
        
                                Heparin plasma(n=5)
                                84-97%
                                90%
                            
        
                        
        
                    
        
                
        
            
        
                        
        
                
        線性
        
                
        
                    
        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        
                    
        
                        
        
                            
        
                                Matrix
                                1:2
                                1:4
                                1:8
                                1:16
                            
        
                            
        
                                serum(n=5)
                                85-94%
                                92-101%
                                87-96%
                                82-90%
                            
        
                            
        
                                EDTA plasma(n=5)
                                83-97%
                                92-103%
                                86-97%
                                93-101%
                            
        
                            
        
                                Heparin plasma(n=5)
                                87-96%
                                86-95%
                                91-103%
                                87-93%
                            
        
                        
        
                    
        
                
        
            
        
                    
        
    
        
    




        
    
        
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