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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
40.00 | 2.075 | 1.994 |
20.00 | 1.647 | 1.566 |
10.00 | 1.282 | 1.201 |
5.00 | 0.933 | 0.852 |
2.50 | 0.468 | 0.387 |
1.25 | 0.426 | 0.345 |
0.63 | 0.226 | 0.145 |
0.00 | 0.081 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 79-93% | 86% |
EDTA plasma(n=5) | 95-107% | 101% |
Heparin plasma(n=5) | 85-95% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-94% | 87-96% | 82-95% | 93-102% |
EDTA plasma(n=5) | 96-103% | 83-96% | 87-98% | 85-97% |
Heparin plasma(n=5) | 78-93% | 96-102% | 95-103% | 89-97% |
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