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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
100.00 | 2.126 | 2.040 |
50.00 | 1.651 | 1.565 |
25.00 | 1.263 | 1.177 |
12.50 | 0.864 | 0.778 |
6.25 | 0.539 | 0.453 |
3.13 | 0.347 | 0.261 |
1.57 | 0.208 | 0.122 |
0.00 | 0.086 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 86-99% | 92% |
EDTA plasma(n=5) | 83-97% | 90% |
Heparin plasma(n=5) | 89-103% | 96% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-104% | 87-96% | 82-96% | 89-103% |
EDTA plasma(n=5) | 85-102% | 81-96% | 87-105% | 83-92% |
Heparin plasma(n=5) | 88-97% | 86-92% | 79-103% | 89-97% |
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