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大鼠分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1548

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Rat sIgA(Secretory Immunoglobulin A) ELISA Kit
Reactivity:	Rat
Alternative Names:	S-IgA
Assay Type:	Sandwich
Sensitivity:	0.072 μg/mL
Standard:	10 μg/mL
Detection Range:	0.16-10 μg/mL
Sample Type:	Serum, plasma,? saliva and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sIgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sIgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sIgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sIgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Open Medicine

Calycosin protects against chronic prostatitis in rats via inhibition of the p38MAPK/NF-κB pathway

日期：2023-07-11 IF：2.1

標(biāo)準(zhǔn)曲線

Concentration (μg/mL)	OD	Corrected OD
10.00	1.941	1.859
5.00	1.554	1.472
2.50	1.117	1.035
1.25	0.735	0.653
0.63	0.456	0.374
0.32	0.389	0.307
0.16	0.236	0.154
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant sIgA and the recovery rates were calculated by comparing the measured value to the expected amount of sIgA in samples.

Matrix	Recovery range	Average
serum(n=5)	83-98%	91%
EDTA plasma(n=5)	91-99%	95%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.