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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
8000.00 | 0.199 | |
4000.00 | 0.397 | |
2000.00 | 0.596 | |
1000.00 | 0.812 | |
500.00 | 1.157 | |
250.00 | 1.592 | |
125.00 | 1.789 | |
0.00 | 2.211 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-99% | 93% |
EDTA plasma(n=5) | 78-90% | 84% |
Heparin plasma(n=5) | 89-107% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 82-94% | 83-96% | 91-103% | 82-99% |
EDTA plasma(n=5) | 78-92% | 85-96% | 93-102% | 95-104% |
Heparin plasma(n=5) | 88-104% | 84-98% | 95-105% | 88-102% |
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