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大鼠趨化因子C-X-C-基元受體3(CXCR3)酶聯(lián)免疫吸附檢測試劑盒

ELK1523

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Rat CXCR3(Chemokine C-X-C-Motif Receptor 3) ELISA Kit
Reactivity:	Rat
Alternative Names:	CD183; CKR-L2; CMKAR3; GPR9; IP10r; IP10-R; Mig-R; MigR; G Protein-Coupled Receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor
Assay Type:	Sandwich
Sensitivity:	0.059 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Uniprot ID:	Q9JII9
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CXCR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CXCR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CXCR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CXCR3 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.219	2.132
5.00	1.643	1.556
2.50	1.107	1.020
1.25	0.821	0.734
0.63	0.518	0.431
0.32	0.364	0.277
0.16	0.206	0.119
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CXCR3 and the recovery rates were calculated by comparing the measured value to the expected amount of CXCR3 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-102%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CXCR3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.