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小鼠腫瘤蛋白p53(TP53)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1508

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Mouse TP53(Tumor Protein p53) ELISA Kit
Reactivity:	Mouse
Alternative Names:	p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Assay Type:	Sandwich
Sensitivity:	2.46 pg/mL
Standard:	400 pg/mL
Detection Range:	6.25-400 pg/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Signal transduction;Metabolic pathway;Tumor immunity;
Uniprot ID:	P02340
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TP53. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TP53 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
400.00	2.214	2.106
200.00	1.782	1.674
100.00	1.277	1.169
50.00	0.935	0.827
25.00	0.563	0.455
12.50	0.395	0.287
6.25	0.251	0.143
0.00	0.108	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TP53 and the recovery rates were calculated by comparing the measured value to the expected amount of TP53 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	96-107%	102%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TP53 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.