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標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 1.997 | 1.896 |
| 5.00 | 1.608 | 1.507 |
| 2.50 | 1.243 | 1.142 |
| 1.25 | 0.896 | 0.795 |
| 0.63 | 0.593 | 0.492 |
| 0.32 | 0.379 | 0.278 |
| 0.16 | 0.226 | 0.125 |
| 0.00 | 0.101 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 96-108% | 102% |
| Heparin plasma(n=5) | 80-95% | 87% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-98% | 92-104% | 82-96% | 95-102% |
| EDTA plasma(n=5) | 89-97% | 86-102% | 94-106% | 85-97% |
| Heparin plasma(n=5) | 96-101% | 85-92% | 79-96% | 89-101% |
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