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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.138 | 2.039 |
10.00 | 1.586 | 1.487 |
5.00 | 1.183 | 1.084 |
2.50 | 0.857 | 0.758 |
1.25 | 0.539 | 0.440 |
0.63 | 0.335 | 0.236 |
0.32 | 0.197 | 0.098 |
0.00 | 0.099 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-105% | 97% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 83-95% | 89% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 79-91% | 89-103% | 82-95% | 86-99% |
EDTA plasma(n=5) | 86-97% | 90-98% | 80-93% | 92-105% |
Heparin plasma(n=5) | 87-98% | 85-92% | 79-96% | 89-100% |
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