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        小鼠誘導型一氧化氮合成酶(NOS2/iNOS)酶聯(lián)免疫吸附檢測試劑盒
        ELK1504
        規(guī)格: 價格:
        48T ¥1960.00
        96T ¥2800.00

        Overview 文獻

        Product name: Mouse NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
        Reactivity: Mouse
        Alternative Names: NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
        Assay Type: Sandwich
        Sensitivity: 0.116 ng/mL
        Standard: 20 ng/mL
        Detection Range: 0.32-20 ng/mL
        Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
        Assay Length: 3.5h
        Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
        Uniprot ID: P29477
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (ng/mL) OD Corrected OD
        20.00 2.138 2.039
        10.00 1.586 1.487
        5.00 1.183 1.084
        2.50 0.857 0.758
        1.25 0.539 0.440
        0.63 0.335 0.236
        0.32 0.197 0.098
        0.00 0.099 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.
        Matrix Recovery range Average
        serum(n=5) 90-105% 97%
        EDTA plasma(n=5) 80-95% 87%
        Heparin plasma(n=5) 83-95% 89%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 79-91% 89-103% 82-95% 86-99%
        EDTA plasma(n=5) 86-97% 90-98% 80-93% 92-105%
        Heparin plasma(n=5) 87-98% 85-92% 79-96% 89-100%
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