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日期：2021-11-02
IF：8.322標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 100.00 | 1.979 | 1.883 |
| 50.00 | 1.562 | 1.466 |
| 25.00 | 1.029 | 0.933 |
| 12.50 | 0.885 | 0.789 |
| 6.25 | 0.561 | 0.465 |
| 3.13 | 0.357 | 0.261 |
| 1.57 | 0.268 | 0.172 |
| 0.00 | 0.096 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 81-97% | 89% |
| EDTA plasma(n=5) | 84-97% | 90% |
| Heparin plasma(n=5) | 82-94% | 88% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-104% | 84-98% | 95-105% | 88-102% |
| EDTA plasma(n=5) | 88-103% | 83-94% | 92-105% | 89-97% |
| Heparin plasma(n=5) | 87-102% | 85-94% | 87-101% | 89-99% |
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