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下載說明 ①Overview
標(biāo)準(zhǔn)曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 1.933 | 1.839 |
| 500.00 | 1.525 | 1.431 |
| 250.00 | 1.028 | 0.934 |
| 125.00 | 0.899 | 0.805 |
| 62.50 | 0.601 | 0.507 |
| 31.25 | 0.345 | 0.251 |
| 15.63 | 0.196 | 0.102 |
| 0.00 | 0.094 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-40 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 93-107% | 100% |
| EDTA plasma(n=5) | 87-99% | 93% |
| Heparin plasma(n=5) | 85-99% | 92% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-97% | 90-99% | 82-101% | 93-105% |
| EDTA plasma(n=5) | 82-95% | 88-102% | 84-97% | 89-107% |
| Heparin plasma(n=5) | 95-105% | 82-95% | 81-97% | 93-105% |
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