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人淀粉樣蛋白β1-40(Ab1-40)酶聯(lián)免疫吸附檢測試劑盒

ELK1492

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
Reactivity:	Human
Alternative Names:	Amyloid Beta Peptide 1-40
Assay Type:	Sandwich
Sensitivity:	4.58 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.082	1.987
500.00	1.621	1.526
250.00	0.997	0.902
125.00	0.765	0.670
62.50	0.503	0.408
31.25	0.351	0.256
15.63	0.221	0.126
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-40 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-94%	87%
EDTA plasma(n=5)	89-101%	95%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.