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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 1.946 | 1.867 |
10.00 | 1.585 | 1.506 |
5.00 | 1.036 | 0.957 |
2.50 | 0.836 | 0.757 |
1.25 | 0.523 | 0.444 |
0.63 | 0.312 | 0.233 |
0.32 | 0.158 | 0.079 |
0.00 | 0.079 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 93-107% | 100% |
EDTA plasma(n=5) | 87-99% | 93% |
Heparin plasma(n=5) | 80-93% | 86% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-90% | 83-96% | 95-102% | 88-95% |
EDTA plasma(n=5) | 83-103% | 82-93% | 86-95% | 81-96% |
Heparin plasma(n=5) | 92-104% | 86-98% | 80-93% | 89-97% |
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