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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
40.00 | 1.956 | 1.869 |
20.00 | 1.578 | 1.491 |
10.00 | 1.123 | 1.036 |
5.00 | 0.827 | 0.740 |
2.50 | 0.573 | 0.486 |
1.25 | 0.387 | 0.300 |
0.63 | 0.215 | 0.128 |
0.00 | 0.087 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 89-107% | 98% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 82-94% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-97% | 87-96% | 85-96% | 85-95% |
EDTA plasma(n=5) | 86-100% | 93-108% | 80-101% | 87-96% |
Heparin plasma(n=5) | 79-96% | 85-99% | 79-93% | 89-97% |
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