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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 2.266 | 2.171 |
250.00 | 1.711 | 1.616 |
125.00 | 1.194 | 1.099 |
62.50 | 0.831 | 0.736 |
31.25 | 0.563 | 0.468 |
15.63 | 0.395 | 0.300 |
7.82 | 0.263 | 0.168 |
0.00 | 0.095 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-97% | 91% |
EDTA plasma(n=5) | 82-96% | 89% |
Heparin plasma(n=5) | 78-90% | 84% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 79-93% | 89-97% | 85-94% | 87-101% |
EDTA plasma(n=5) | 86-95% | 93-103% | 87-98% | 85-96% |
Heparin plasma(n=5) | 91-99% | 88-97% | 88-104% | 97-108% |
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