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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
50.00 | 2.195 | 2.110 |
25.00 | 1.657 | 1.572 |
12.50 | 1.182 | 1.097 |
6.25 | 0.933 | 0.848 |
3.13 | 0.589 | 0.504 |
1.57 | 0.315 | 0.230 |
0.79 | 0.189 | 0.104 |
0.00 | 0.085 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 81-103% | 87% |
EDTA plasma(n=5) | 93-105% | 99% |
Heparin plasma(n=5) | 84-93% | 89% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 82-93% | 95-104% | 82-96% | 85-98% |
EDTA plasma(n=5) | 95-104% | 82-96% | 92-105% | 83-92% |
Heparin plasma(n=5) | 87-92% | 85-94% | 89-98% | 85-94% |
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