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下載說明 ①Overview
標準曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 500.00 | 2.172 | 2.074 |
| 250.00 | 1.588 | 1.490 |
| 125.00 | 1.106 | 1.008 |
| 62.50 | 0.835 | 0.737 |
| 31.25 | 0.539 | 0.441 |
| 15.63 | 0.384 | 0.286 |
| 7.82 | 0.247 | 0.149 |
| 0.00 | 0.098 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant IL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-37 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 80-97% | 88% |
| EDTA plasma(n=5) | 83-95% | 89% |
| Heparin plasma(n=5) | 81-95% | 88% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-102% | 93-105% | 91-99% | 83-96% |
| EDTA plasma(n=5) | 79-91% | 87-96% | 95-103% | 85-91% |
| Heparin plasma(n=5) | 85-92% | 90-96% | 95-102% | 87-98% |
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