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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.043 | 1.957 |
10.00 | 1.527 | 1.441 |
5.00 | 1.263 | 1.177 |
2.50 | 0.864 | 0.778 |
1.25 | 0.539 | 0.453 |
0.63 | 0.347 | 0.261 |
0.32 | 0.208 | 0.122 |
0.00 | 0.086 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-105% | 97% |
EDTA plasma(n=5) | 80-97% | 88% |
Heparin plasma(n=5) | 83-95% | 89% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-102% | 91-99% | 83-95% | 89-97% |
EDTA plasma(n=5) | 85-94% | 78-96% | 79-93% | 82-90% |
Heparin plasma(n=5) | 95-103% | 89-101% | 87-98% | 85-92% |
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