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小鼠血管內(nèi)皮生長因子受體2(VEGFR2)酶聯(lián)免疫吸附檢測試劑盒

ELK1287

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Mouse VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1
Assay Type:	Sandwich
Sensitivity:	0.058 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VEGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Gut microbiota modulation and effects of a diet enriched in apple pomace on inflammation in a DSS-induced colitis mouse model

Food&Function

日期：2024-02-07 IF：6.1

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.204	2.096
5.00	1.608	1.500
2.50	1.282	1.174
1.25	0.864	0.756
0.63	0.509	0.401
0.32	0.387	0.279
0.16	0.226	0.118
0.00	0.108	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VEGFR2 and the recovery rates were calculated by comparing the measured value to the expected amount of VEGFR2 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.