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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
5000.00 | 1.948 | 1.844 |
2500.00 | 1.593 | 1.489 |
1250.00 | 1.116 | 1.012 |
625.00 | 0.705 | 0.601 |
312.50 | 0.468 | 0.364 |
156.25 | 0.308 | 0.204 |
78.13 | 0.256 | 0.152 |
0.00 | 0.104 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-95% | 88% |
EDTA plasma(n=5) | 95-107% | 101% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-101% | 79-92% | 82-93% | 83-97% |
EDTA plasma(n=5) | 82-103% | 81-91% | 85-101% | 81-90% |
Heparin plasma(n=5) | 85-98% | 81-96% | 87-98% | 92-103% |
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