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小鼠腸脂肪酸結(jié)合蛋白(IFABP/FABP2)酶聯(lián)免疫吸附檢測試劑盒

ELK1265

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview

Product name:	Mouse IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
Reactivity:	Mouse
Alternative Names:	FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;
Uniprot ID:	P55050
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.201	2.105
5.00	1.647	1.551
2.50	1.186	1.090
1.25	0.822	0.726
0.63	0.515	0.419
0.32	0.388	0.292
0.16	0.234	0.138
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IFABP/FABP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFABP/FABP2 in samples.

Matrix	Recovery range	Average
serum(n=5)	96-109%	102%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	85-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFABP/FABP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.