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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.389 | 2.308 |
500.00 | 1.436 | 1.355 |
250.00 | 0.947 | 0.866 |
125.00 | 0.625 | 0.544 |
62.50 | 0.493 | 0.412 |
31.25 | 0.352 | 0.271 |
15.63 | 0.214 | 0.133 |
0.00 | 0.081 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-96% | 89% |
EDTA plasma(n=5) | 92-107% | 99% |
Heparin plasma(n=5) | 88-102% | 95% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 81-94% | 96-105% | 92-103% | 82-96% |
EDTA plasma(n=5) | 86-97% | 83-96% | 91-103% | 96-105% |
Heparin plasma(n=5) | 86-93% | 91-105% | 87-98% | 89-94% |
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