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        小鼠β細(xì)胞素(bTC)酶聯(lián)免疫吸附檢測試劑盒
        ELK1236
        規(guī)格: 價(jià)格:
        48T ¥1680.00
        96T ¥2400.00

        Overview

        Product name: Mouse bTC(Betacellulin) ELISA Kit
        Reactivity: Mouse
        Alternative Names: Probetacellulin
        Assay Type: Sandwich
        Sensitivity: 6.4 pg/mL
        Standard: 1000 pg/mL
        Detection Range: 15.63-1000 pg/mL
        Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
        Assay Length: 3.5h
        Research Area: Cytokine;Tumor immunity;
        Uniprot ID: Q05928
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse bTC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bTC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse bTC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bTC in the samples is then determined by comparing the OD of the samples to the standard curve.

        標(biāo)準(zhǔn)曲線

        Concentration (pg/mL) OD Corrected OD
        1000.00 2.389 2.308
        500.00 1.436 1.355
        250.00 0.947 0.866
        125.00 0.625 0.544
        62.50 0.493 0.412
        31.25 0.352 0.271
        15.63 0.214 0.133
        0.00 0.081 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant bTC and the recovery rates were calculated by comparing the measured value to the expected amount of bTC in samples.
        Matrix Recovery range Average
        serum(n=5) 83-96% 89%
        EDTA plasma(n=5) 92-107% 99%
        Heparin plasma(n=5) 88-102% 95%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of bTC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 81-94% 96-105% 92-103% 82-96%
        EDTA plasma(n=5) 86-97% 83-96% 91-103% 96-105%
        Heparin plasma(n=5) 86-93% 91-105% 87-98% 89-94%
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