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下載說明 ①Overview 文獻
日期：2023-07-01
IF：2.4標準曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 2500.00 | 2.037 | 1.951 |
| 1250.00 | 1.572 | 1.486 |
| 625.00 | 1.216 | 1.130 |
| 312.50 | 0.921 | 0.835 |
| 156.25 | 0.563 | 0.477 |
| 78.13 | 0.403 | 0.317 |
| 39.07 | 0.256 | 0.170 |
| 0.00 | 0.086 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-103% | 96% |
| EDTA plasma(n=5) | 81-96% | 88% |
| Heparin plasma(n=5) | 78-94% | 86% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 82-94% | 95-102% | 87-103% | 88-95% |
| EDTA plasma(n=5) | 98-107% | 90-99% | 88-97% | 85-97% |
| Heparin plasma(n=5) | 81-94% | 87-105% | 79-95% | 96-105% |
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