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人b2微球蛋白(BMG/b2-MG)酶聯(lián)免疫吸附檢測試劑盒

ELK1200

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Human BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity:	Human
Alternative Names:	Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type:	Sandwich
Sensitivity:	0.16 ng/mL
Standard:	25 ng/mL
Detection Range:	0.4-25 ng/mL
Sample Type:	serum, plasma, urine and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Kidney biomarker;
Uniprot ID:	P61769
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

KIDNEY & BLOOD PRESSURE RESEARCH

Relationship between Plasma Trimethylamine N-Oxide Levels and Renal Dysfunction in Patients with Hypertension

日期：2021-08-04 IF：2.687

標準曲線

Concentration (ng/mL)	OD	Corrected OD
25.00	2.201	2.117
12.50	1.593	1.509
6.25	1.121	1.037
3.13	0.892	0.808
1.57	0.547	0.463
0.79	0.345	0.261
0.40	0.265	0.181
0.00	0.084	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant BMG/b2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of BMG/b2-MG in samples.

Matrix	Recovery range	Average
serum(n=5)	78-91%	84%
EDTA plasma(n=5)	88-101%	94%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMG/b2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.