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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1250.00 | 2.075 | 1.992 |
625.00 | 1.502 | 1.419 |
312.50 | 1.174 | 1.091 |
156.25 | 0.797 | 0.714 |
78.13 | 0.532 | 0.449 |
39.07 | 0.319 | 0.236 |
19.54 | 0.179 | 0.096 |
0.00 | 0.083 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 91-105% | 98% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 88-101% | 94% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 83-96% | 93-102% | 87-98% |
EDTA plasma(n=5) | 92-103% | 93-105% | 88-101% | 97-106% |
Heparin plasma(n=5) | 85-94% | 90-99% | 95-102% | 82-90% |
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