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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 1.952 | 1.884 |
500.00 | 1.387 | 1.319 |
250.00 | 0.939 | 0.871 |
125.00 | 0.752 | 0.684 |
62.50 | 0.553 | 0.485 |
31.25 | 0.329 | 0.261 |
15.63 | 0.142 | 0.074 |
0.00 | 0.068 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-95% | 88% |
EDTA plasma(n=5) | 80-93% | 86% |
Heparin plasma(n=5) | 95-108% | 101% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 80-92% | 86-102% | 87-103% | 85-101% |
EDTA plasma(n=5) | 82-93% | 96-105% | 87-98% | 85-97% |
Heparin plasma(n=5) | 82-94% | 85-96% | 78-90% | 91-99% |
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