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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 2.268 | 2.172 |
250.00 | 1.647 | 1.551 |
125.00 | 0.997 | 0.901 |
62.50 | 0.682 | 0.586 |
31.25 | 0.395 | 0.299 |
15.63 | 0.315 | 0.219 |
7.82 | 0.226 | 0.130 |
0.00 | 0.096 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-95% | 91% |
EDTA plasma(n=5) | 81-95% | 88% |
Heparin plasma(n=5) | 83-97% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 92-104% | 82-96% | 95-102% | 87-98% |
EDTA plasma(n=5) | 90-99% | 83-97% | 81-103% | 89-101% |
Heparin plasma(n=5) | 79-91% | 89-103% | 82-95% | 86-99% |
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