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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
100.00 | 2.172 | 2.099 |
50.00 | 1.720 | 1.647 |
25.00 | 1.223 | 1.150 |
12.50 | 0.682 | 0.609 |
6.25 | 0.639 | 0.566 |
3.13 | 0.354 | 0.281 |
1.57 | 0.251 | 0.178 |
0.00 | 0.073 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-95% | 90% |
EDTA plasma(n=5) | 79-93% | 86% |
Heparin plasma(n=5) | 95-107% | 101% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-98% | 92-105% | 95-107% | 82-96% |
EDTA plasma(n=5) | 83-97% | 86-97% | 91-99% | 95-103% |
Heparin plasma(n=5) | 95-104% | 93-102% | 79-94% | 87-98% |
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