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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.058 | 1.990 |
500.00 | 1.587 | 1.519 |
250.00 | 1.069 | 1.001 |
125.00 | 0.797 | 0.729 |
62.50 | 0.532 | 0.464 |
31.25 | 0.364 | 0.296 |
15.63 | 0.206 | 0.138 |
0.00 | 0.068 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 93-105% | 99% |
EDTA plasma(n=5) | 84-93% | 89% |
Heparin plasma(n=5) | 81-103% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 91-99% | 83-89% | 88-104% | 97-103% |
EDTA plasma(n=5) | 85-95% | 79-96% | 89-101% | 85-97% |
Heparin plasma(n=5) | 86-96% | 93-104% | 89-97% | 85-94% |
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