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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 1.984 | 1.916 |
5.00 | 1.593 | 1.525 |
2.50 | 1.135 | 1.067 |
1.25 | 0.782 | 0.714 |
0.63 | 0.514 | 0.446 |
0.32 | 0.321 | 0.253 |
0.16 | 0.225 | 0.157 |
0.00 | 0.068 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 86-99% | 92% |
Heparin plasma(n=5) | 83-95% | 89% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-99% | 83-99% | 98-105% | 96-101% |
EDTA plasma(n=5) | 85-92% | 79-96% | 89-101% | 85-97% |
Heparin plasma(n=5) | 86-94% | 93-102% | 89-97% | 85-94% |
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