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        人基質(zhì)金屬蛋白酶8(MMP8)酶聯(lián)免疫吸附檢測試劑盒
        ELK1172
        規(guī)格: 價(jià)格:
        48T ¥1680.00
        96T ¥2400.00

        Overview 文獻(xiàn)

        Product name: Human MMP8(Matrix Metalloproteinase 8) ELISA Kit
        Reactivity: Human
        Alternative Names: CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase
        Assay Type: Sandwich
        Sensitivity: 28 pg/mL
        Standard: 5000 pg/mL
        Detection Range: 78.13-5000 pg/mL
        Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
        Assay Length: 3.5h
        Research Area: Enzyme & Kinase;Infection immunity;Cardiovascular biology;
        Uniprot ID: P22894
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP8 in the samples is then determined by comparing the OD of the samples to the standard curve.

        標(biāo)準(zhǔn)曲線

        Concentration (pg/mL) OD Corrected OD
        5000.00 2.027 1.954
        2500.00 1.497 1.424
        1250.00 1.069 0.996
        625.00 0.883 0.810
        312.50 0.559 0.486
        156.25 0.303 0.230
        78.13 0.232 0.159
        0.00 0.073 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant MMP8 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP8 in samples.
        Matrix Recovery range Average
        serum(n=5) 90-105% 97%
        EDTA plasma(n=5) 80-95% 87%
        Heparin plasma(n=5) 86-99% 92%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 86-97% 88-95% 92-101% 87-98%
        EDTA plasma(n=5) 92-101% 82-98% 89-97% 85-93%
        Heparin plasma(n=5) 86-101% 86-101% 87-96% 92-105%
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