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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
40.00 | 2.146 | 2.051 |
20.00 | 1.565 | 1.470 |
10.00 | 1.075 | 0.980 |
5.00 | 0.975 | 0.880 |
2.50 | 0.567 | 0.472 |
1.25 | 0.388 | 0.293 |
0.63 | 0.197 | 0.102 |
0.00 | 0.095 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 78-92% | 85% |
EDTA plasma(n=5) | 87-99% | 93% |
Heparin plasma(n=5) | 85-99% | 92% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 91-99% | 88-97% | 88-104% | 97-108% |
EDTA plasma(n=5) | 79-93% | 89-97% | 85-94% | 87-101% |
Heparin plasma(n=5) | 86-95% | 93-103% | 87-98% | 85-96% |
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