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小鼠腎損傷分子1(Kim1)酶聯(lián)免疫吸附檢測試劑盒

ELK1165

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse Kim1(Kidney Injury Molecule 1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1
Assay Type:	Sandwich
Sensitivity:	15 pg/mL
Standard:	2500 pg/mL
Detection Range:	39.07-2500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluidstissue homogenates
Assay Length:	3.5h
Research Area:	Tumor immunity;Infection immunity;Kidney biomarker;
Uniprot ID:	Q5QNS5
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Kim1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Kim1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Kim1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Kim1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Diabetes, Metabolic Syndrome and Obesity

Jiangtang Decoction Ameliorates Diabetic Kidney Disease Through the Modulation of the Gut Microbiota

日期：2023-11-07 IF：3.3

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2500.00	2.252	2.101
1250.00	1.738	1.587
625.00	1.224	1.073
312.50	0.907	0.756
156.25	0.593	0.442
78.13	0.354	0.203
39.07	0.268	0.117
0.00	0.151	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Kim1 and the recovery rates were calculated by comparing the measured value to the expected amount of Kim1 in samples.

Matrix	Recovery range	Average
serum(n=5)	93-107%	100%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kim1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.