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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2500.00 | 2.252 | 2.101 |
1250.00 | 1.738 | 1.587 |
625.00 | 1.224 | 1.073 |
312.50 | 0.907 | 0.756 |
156.25 | 0.593 | 0.442 |
78.13 | 0.354 | 0.203 |
39.07 | 0.268 | 0.117 |
0.00 | 0.151 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 93-107% | 100% |
EDTA plasma(n=5) | 87-95% | 91% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 80-101% | 87-96% | 96-105% | 95-103% |
EDTA plasma(n=5) | 85-105% | 87-98% | 91-102% | 86-102% |
Heparin plasma(n=5) | 86-94% | 93-102% | 87-96% | 92-105% |
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