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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.121 | 2.023 |
5.00 | 1.694 | 1.596 |
2.50 | 1.177 | 1.079 |
1.25 | 0.847 | 0.749 |
0.63 | 0.625 | 0.527 |
0.32 | 0.384 | 0.286 |
0.16 | 0.238 | 0.140 |
0.00 | 0.098 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 92-106% | 99% |
EDTA plasma(n=5) | 77-95% | 86% |
Heparin plasma(n=5) | 88-103% | 95% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-96% | 92-105% | 89-102% | 92-101% |
EDTA plasma(n=5) | 79-93% | 89-97% | 85-94% | 87-101% |
Heparin plasma(n=5) | 86-94% | 85-95% | 89-99% | 85-95% |
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