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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.054 | 1.959 |
500.00 | 1.624 | 1.529 |
250.00 | 1.219 | 1.124 |
125.00 | 0.907 | 0.812 |
62.50 | 0.587 | 0.492 |
31.25 | 0.325 | 0.230 |
15.63 | 0.193 | 0.098 |
0.00 | 0.095 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-95% | 89% |
EDTA plasma(n=5) | 81-95% | 88% |
Heparin plasma(n=5) | 80-97% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-104% | 82-94% | 86-96% | 81-97% |
EDTA plasma(n=5) | 92-107% | 83-99% | 80-96% | 91-101% |
Heparin plasma(n=5) | 85-97% | 86-102% | 87-99% | 99-105% |
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