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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 1.952 | 1.857 |
1000.00 | 1.387 | 1.292 |
500.00 | 0.913 | 0.818 |
250.00 | 0.847 | 0.752 |
125.00 | 0.474 | 0.379 |
62.50 | 0.302 | 0.207 |
31.25 | 0.196 | 0.101 |
0.00 | 0.095 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-96% | 88% |
EDTA plasma(n=5) | 80-92% | 86% |
Heparin plasma(n=5) | 78-95% | 86% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-95% | 85-96% | 89-100% | 85-96% |
EDTA plasma(n=5) | 85-94% | 87-101% | 86-97% | 88-95% |
Heparin plasma(n=5) | 92-101% | 87-98% | 85-92% | 79-96% |
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