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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
5000.00 | 2.121 | 2.025 |
2500.00 | 1.674 | 1.578 |
1250.00 | 1.144 | 1.048 |
625.00 | 0.975 | 0.879 |
312.50 | 0.593 | 0.497 |
156.25 | 0.315 | 0.219 |
78.13 | 0.226 | 0.130 |
0.00 | 0.096 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 86-99% | 92% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 83-98% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-95% | 85-97% | 89-101% | 85-97% |
EDTA plasma(n=5) | 80-101% | 87-96% | 96-105% | 95-103% |
Heparin plasma(n=5) | 95-103% | 87-98% | 85-92% | 79-96% |
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