• <th id="abimu"></th>

      1. 產品中心

        人葡萄糖激酶(GCK)酶聯(lián)免疫吸附檢測試劑盒
        ELK1111
        規(guī)格: 價格:
        48T ¥1680.00
        96T ¥2400.00

        Overview

        Product name: Human GCK(Glucokinase) ELISA Kit
        Reactivity: Human
        Alternative Names: GK; GLK; HHF3; HK4; HKIV; HXKP; MODY2; Hexokinase-D; Hexokinase 4; Maturity Onset Diabetes Of The Young 2
        Assay Type: Sandwich
        Sensitivity: 0.23 ng/mL
        Standard: 50 ng/mL
        Detection Range: 0.79-50 ng/mL
        Sample Type: Tissue homogenates and other biological fluids.
        Assay Length: 3.5h
        Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;
        Uniprot ID: P35557
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GCK in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (ng/mL) OD Corrected OD
        50.00 2.388 2.235
        25.00 1.649 1.496
        12.50 1.240 1.087
        6.25 0.797 0.644
        3.13 0.474 0.321
        1.57 0.302 0.149
        0.79 0.287 0.134
        0.00 0.153 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant GCK and the recovery rates were calculated by comparing the measured value to the expected amount of GCK in samples.
        Matrix Recovery range Average
        serum(n=5) 80-97% 88%
        EDTA plasma(n=5) 83-95% 89%
        Heparin plasma(n=5) 90-105% 97%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 85-98% 88-104% 86-102% 79-93%
        EDTA plasma(n=5) 89-97% 85-94% 87-101% 85-96%
        Heparin plasma(n=5) 85-94% 87-101% 82-95% 82-93%
        關閉

        在線咨詢

        Online consultation

        • 在線咨詢
        • 技術支持

        關注微信公眾號

        微信掃一掃立即咨詢

        微信掃一掃立即咨詢

        關閉
        一级a爱片国产亚洲精品,97se亚洲综合色区,日朝欧美亚洲精品,欧洲韩国亚洲一区二区三区

      2. <th id="abimu"></th>