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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
50.00 | 2.388 | 2.235 |
25.00 | 1.649 | 1.496 |
12.50 | 1.240 | 1.087 |
6.25 | 0.797 | 0.644 |
3.13 | 0.474 | 0.321 |
1.57 | 0.302 | 0.149 |
0.79 | 0.287 | 0.134 |
0.00 | 0.153 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-97% | 88% |
EDTA plasma(n=5) | 83-95% | 89% |
Heparin plasma(n=5) | 90-105% | 97% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-98% | 88-104% | 86-102% | 79-93% |
EDTA plasma(n=5) | 89-97% | 85-94% | 87-101% | 85-96% |
Heparin plasma(n=5) | 85-94% | 87-101% | 82-95% | 82-93% |
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