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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 1.916 | 1.818 |
500.00 | 1.527 | 1.429 |
250.00 | 1.186 | 1.088 |
125.00 | 0.872 | 0.774 |
62.50 | 0.548 | 0.450 |
31.25 | 0.315 | 0.217 |
15.63 | 0.226 | 0.128 |
0.00 | 0.098 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 96-108% | 102% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 79-96% | 95-103% | 87-98% |
EDTA plasma(n=5) | 88-97% | 96-103% | 86-92% | 89-97% |
Heparin plasma(n=5) | 80-101% | 87-96% | 96-105% | 95-103% |
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