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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 2.013 | 1.918 |
1000.00 | 1.616 | 1.521 |
500.00 | 1.235 | 1.140 |
250.00 | 0.907 | 0.812 |
125.00 | 0.632 | 0.537 |
62.50 | 0.389 | 0.294 |
31.25 | 0.257 | 0.162 |
0.00 | 0.095 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-94% | 88% |
EDTA plasma(n=5) | 81-97% | 89% |
Heparin plasma(n=5) | 84-97% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 87-101% | 88-102% | 79-102% |
EDTA plasma(n=5) | 88-97% | 96-105% | 86-92% | 89-97% |
Heparin plasma(n=5) | 85-94% | 79-96% | 92-101% | 82-90% |
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