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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 1.983 | 1.898 |
250.00 | 1.615 | 1.530 |
125.00 | 1.052 | 0.967 |
62.50 | 0.697 | 0.612 |
31.25 | 0.541 | 0.456 |
15.63 | 0.329 | 0.244 |
7.82 | 0.208 | 0.123 |
0.00 | 0.085 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-97% | 90% |
EDTA plasma(n=5) | 81-95% | 88% |
Heparin plasma(n=5) | 85-95% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 83-92% | 85-96% | 93-99% | 95-102% |
EDTA plasma(n=5) | 80-97% | 81-92% | 87-103% | 82-93% |
Heparin plasma(n=5) | 95-104% | 82-96% | 92-105% | 83-92% |
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