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人血小板衍生生長因子AB(PDGFAB)酶聯(lián)免疫吸附檢測試劑盒

ELK1089

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Human PDGFAB(Platelet Derived Growth Factor AB) ELISA Kit
Reactivity:	Human
Alternative Names:	PDGF-AB
Assay Type:	Sandwich
Sensitivity:	27 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Hepatology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDGFAB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFAB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDGFAB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDGFAB in the samples is then determined by comparing the OD of the samples to the standard curve.

Biomedicines

Comparison between Platelet Lysate, Platelet Lysate Serum, and Fetal Bovine Serum as Supplements for Cell Culture, Expansion, and Cryopreservation

日期：2024-01-02 IF：4.7

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	1.952	1.868
2500.00	1.428	1.344
1250.00	1.067	0.983
625.00	0.756	0.672
312.50	0.435	0.351
156.25	0.279	0.195
78.13	0.157	0.073
0.00	0.084	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PDGFAB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFAB in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	80-94%	87%
Heparin plasma(n=5)	89-101%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFAB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.